Journal: Cancer Science

Article Title: Design and Evaluation of Eb 4 Mab ‐7‐ mG 2a : A Dual‐Action Anti‐ EphB4 Monoclonal Antibody for Targeted Breast Cancer Therapy

doi: 10.1111/cas.70198

Figure Lengend Snippet: Prognostic significance of EphB4 expression in breast cancer and establishment of Eb 4 Mab‐7‐mG 2a . (A) Kaplan–Meier survival analysis of OS in breast cancer patients based on the expression levels of EphB family genes (EphB1, EphB2, EphB3, EphB4, and EphB6) using RNA‐seq data from KMplot. Among these genes, high expression of EphB2 and EphB4 was significantly associated with poor prognosis (log‐rank p < 0.05). Patients were then stratified into high and low expression groups using the optimal cutoff within the interquartile range. (B) Generation of a mouse IgG 2a version of the anti‐EphB4 monoclonal antibody (Eb 4 Mab‐7‐mG 2a ). The V H and V L regions of B4Mab‐7 (mouse IgG 1 ) were cloned into expression vectors containing mouse IgG 2a C H and C L regions. These vectors were transfected into BINDS‐09 cells to produce Eb 4 Mab‐7‐mG 2a . (C) Left: Expression of EphB4 and β‐Actin in CHO‐K1, CHO/EphB4, MCF‐7, and EphB4‐KO MCF‐7 cells. Right: Expression of EphB4 and GAPDH in normal human adult breast tissues, MCF10A (non‐tumorigenic mammary epithelial cells), MCF‐7, BT‐474, SK‐BR‐3, MDA‐MB‐468, and HBC5 cells.

Article Snippet: Aside from cell lysates, a commercially available normal human breast tissue lysate (Total Protein–Human Adult Normal Tissue: Breast, Novus Biologicals, Littleton, CO, USA) was used to assess expression in normal breast tissue.

Techniques: Expressing, RNA Sequencing, Clone Assay, Transfection

Journal: bioRxiv

Article Title: Direct measurement of RNA Polymerase II hypertranscription in cancer FFPE samples

doi: 10.1101/2024.02.28.582647

Figure Lengend Snippet: Pathology classification, age and sex were provided by the vendor (BioChain). Each image spans the width of a standard charged microscope slide, where the tissue is visible under the paraffin skin. On-slide RNAPII-Ser5p FFPE-CUTAC was applied to slides in parallel, using a total of four slides each for 100 separate samples in all to produce the data analyzed in this study.

Article Snippet: The following pairs of human tumor and adjacent normal 5 μm tissue sections from single FFPE blocks were purchased from Biochain, Inc: Breast Normal/Tumor cat. no. T8235086PP/PT; Colon Normal/Tumor cat. no. T8235090PP/PT; Kidney Normal/Tumor cat. no. T8235142PP/PT; Liver Normal/Tumor cat. no. T8235149PP/PT; Lung Normal/Tumor cat. no. T8235152PP/PT; Rectum Normal/Tumor cat. no. T8235206PP/PT; Stomach Normal/Tumor cat. no. T8235248PP/PT.

Techniques: Microscopy

Journal: Molecular Cancer

Article Title: Survivin 2α: a novel Survivin splice variant expressed in human malignancies

doi: 10.1186/1476-4598-4-11

Figure Lengend Snippet: Survivin 2α expression (relative to normal tissue).

Article Snippet: Seven fresh frozen primary medulloblastoma tumor samples were obtained from the Cooperative Human Tumor Network (CHTN), after approval through the Columbus Children's Hospital IRB.

Techniques: Expressing

Journal: Molecular Cancer

Article Title: Survivin 2α: a novel Survivin splice variant expressed in human malignancies

doi: 10.1186/1476-4598-4-11

Figure Lengend Snippet: Expression of survivin splice variants in medulloblastoma (relative to survivin)

Article Snippet: Seven fresh frozen primary medulloblastoma tumor samples were obtained from the Cooperative Human Tumor Network (CHTN), after approval through the Columbus Children's Hospital IRB.

Techniques: Expressing